Characterization and Analysis of the Composition and Dynamics of the Mammalian Riboproteome
Increasing evidence points to an important role for the ribosome in the regulation of biological processes and as a target for deregulation in disease.Here, we describe a SILAC (stable isotope labeling by amino acids in cell culture)-based mass spectrometry approach to probing mammalian riboproteomes.Using a panel of cell lines, as well as genetic